diff --git a/packages/playwright/src/matchers/matchers.ts b/packages/playwright/src/matchers/matchers.ts
index c9f3a8f3de..3962c0cae9 100644
--- a/packages/playwright/src/matchers/matchers.ts
+++ b/packages/playwright/src/matchers/matchers.ts
@@ -212,7 +212,7 @@ export function toHaveAccessibleErrorMessage(
   options?: { timeout?: number; ignoreCase?: boolean },
 ) {
   return toMatchText.call(this, 'toHaveAccessibleErrorMessage', locator, 'Locator', async (isNot, timeout) => {
-    const expectedText = serializeExpectedTextValues([expected], { ignoreCase: options?.ignoreCase });
+    const expectedText = serializeExpectedTextValues([expected], { ignoreCase: options?.ignoreCase, normalizeWhiteSpace: true });
     return await locator._expect('to.have.accessible.error.message', { expectedText: expectedText, isNot, timeout });
   }, expected, options);
 }