diff --git a/packages/playwright-core/src/server/trace/viewer/traceViewer.ts b/packages/playwright-core/src/server/trace/viewer/traceViewer.ts
index d053a3e643..6ca0319aa3 100644
--- a/packages/playwright-core/src/server/trace/viewer/traceViewer.ts
+++ b/packages/playwright-core/src/server/trace/viewer/traceViewer.ts
@@ -109,6 +109,8 @@ export async function startTraceViewerServer(options?: TraceViewerServerOptions)
 
 export async function installRootRedirect(server: HttpServer, traceUrls: string[], options: TraceViewerRedirectOptions) {
   const params = new URLSearchParams();
+  if (path.sep !== path.posix.sep)
+    params.set('pathSeparator', path.sep);
   for (const traceUrl of traceUrls)
     params.append('trace', traceUrl);
   if (server.wsGuid())
diff --git a/packages/trace-viewer/src/ui/uiModeTraceView.tsx b/packages/trace-viewer/src/ui/uiModeTraceView.tsx
index e34ab6aede..8027005812 100644
--- a/packages/trace-viewer/src/ui/uiModeTraceView.tsx
+++ b/packages/trace-viewer/src/ui/uiModeTraceView.tsx
@@ -31,7 +31,8 @@ export const TraceView: React.FC<{
   rootDir?: string,
   onOpenExternally?: (location: SourceLocation) => void,
   revealSource?: boolean,
-}> = ({ item, rootDir, onOpenExternally, revealSource }) => {
+  pathSeparator: string,
+}> = ({ item, rootDir, onOpenExternally, revealSource, pathSeparator }) => {
   const [model, setModel] = React.useState<{ model: MultiTraceModel, isLive: boolean } | undefined>();
   const [counter, setCounter] = React.useState(0);
   const pollTimer = React.useRef<NodeJS.Timeout | null>(null);
@@ -69,7 +70,12 @@ export const TraceView: React.FC<{
       return;
     }
 
-    const traceLocation = `${outputDir}/${artifactsFolderName(result!.workerIndex)}/traces/${item.testCase?.id}.json`;
+    const traceLocation = [
+      outputDir,
+      artifactsFolderName(result!.workerIndex),
+      'traces',
+      `${item.testCase?.id}.json`
+    ].join(pathSeparator);
     // Start polling running test.
     pollTimer.current = setTimeout(async () => {
       try {
diff --git a/packages/trace-viewer/src/ui/uiModeView.tsx b/packages/trace-viewer/src/ui/uiModeView.tsx
index cad1c2eb58..2c4b61fdad 100644
--- a/packages/trace-viewer/src/ui/uiModeView.tsx
+++ b/packages/trace-viewer/src/ui/uiModeView.tsx
@@ -38,8 +38,6 @@ import { TestListView } from './uiModeTestListView';
 import { TraceView } from './uiModeTraceView';
 import { SettingsView } from './settingsView';
 
-const pathSeparator = navigator.userAgent.toLowerCase().includes('windows') ? '\\' : '/';
-
 let xtermSize = { cols: 80, rows: 24 };
 const xtermDataSource: XtermDataSource = {
   pending: [],
@@ -63,6 +61,7 @@ const queryParams = {
   outputDir: searchParams.get('outputDir') || undefined,
   updateSnapshots: (searchParams.get('updateSnapshots') as 'all' | 'none' | 'missing' | undefined) || undefined,
   reporters: searchParams.has('reporter') ? searchParams.getAll('reporter') : undefined,
+  pathSeparator: searchParams.get('pathSeparator') || '/',
 };
 if (queryParams.updateSnapshots && !['all', 'none', 'missing'].includes(queryParams.updateSnapshots))
   queryParams.updateSnapshots = undefined;
@@ -170,7 +169,7 @@ export const UIModeView: React.FC<{}> = ({
       onError: error => {
         xtermDataSource.write((error.stack || error.value || '') + '\n');
       },
-      pathSeparator,
+      pathSeparator: queryParams.pathSeparator,
     });
 
     setTeleSuiteUpdater(teleSuiteUpdater);
@@ -242,8 +241,8 @@ export const UIModeView: React.FC<{}> = ({
   // Test tree is built from the model and filters.
   const { testTree } = React.useMemo(() => {
     if (!testModel)
-      return { testTree: new TestTree('', new TeleSuite('', 'root'), [], projectFilters, pathSeparator) };
-    const testTree = new TestTree('', testModel.rootSuite, testModel.loadErrors, projectFilters, pathSeparator);
+      return { testTree: new TestTree('', new TeleSuite('', 'root'), [], projectFilters, queryParams.pathSeparator) };
+    const testTree = new TestTree('', testModel.rootSuite, testModel.loadErrors, projectFilters, queryParams.pathSeparator);
     testTree.filterTree(filterText, statusFilters, isRunningTest ? runningState?.testIds : undefined);
     testTree.sortAndPropagateStatus();
     testTree.shortenRoot();
@@ -332,7 +331,7 @@ export const UIModeView: React.FC<{}> = ({
 
       // run affected watched tests
       const testModel = teleSuiteUpdater.asModel();
-      const testTree = new TestTree('', testModel.rootSuite, testModel.loadErrors, projectFilters, pathSeparator);
+      const testTree = new TestTree('', testModel.rootSuite, testModel.loadErrors, projectFilters, queryParams.pathSeparator);
 
       const testIds: string[] = [];
       const set = new Set(params.testFiles);
@@ -435,6 +434,7 @@ export const UIModeView: React.FC<{}> = ({
         </div>
         <div className={clsx('vbox', isShowingOutput && 'hidden')}>
           <TraceView
+            pathSeparator={queryParams.pathSeparator}
             item={selectedItem}
             rootDir={testModel?.config?.rootDir}
             revealSource={revealSource}